Yang Yang, Yun Liu, Qiumei Shi, Zhiqiang Zhang, Tonglei Wu, Guoqiang Zhu
Jiangsu Co-Innovation Centerfor for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China (Yang Yang, Yun Liu, Guoqiang Zhu); Hebei Normal University of Science & Technology, Key Laboratory of Hebei Province Preventive Veterinary Medicine, Qinhuangdao, 066004, China (Qiumei Shi, Zhiqiang Zhang, Tonglei Wu)
Guoqiang Zhu (E-mail: email@example.com, firstname.lastname@example.org)
Bacteriology; Infectious diseases; Inorganic chemistry; Microbiology; Pharmacology; Veterinary medicine
Low-concentration dietary copper has been widely used for domestic animals worldwide. To investigate the potential antimicrobial effect of low-concentration of copper, a pathogenic Shiga-like toxigenic Escherichia coli (SLTEC) strain, 107/86, cultured in the medium complemented with 1.0 mmol/L copper, was examined regarding alteration of certain virulence factors. The results showed that, compared to the untreated control strain, the Cu-treated strain 107/86 displayed suppressed growth rate, impaired adherence to IPEC-J2 cells, and decreased stx2e production. Reduced adherence to IPEC-J2 cells was confirmed using a real-time RT-PCR assay which targeted adhesion-associated genes. Additionally, the Cu-treated 107/86 differed from the untreated control with 33-fold lower activity of AI-2 in the quorum sensing system as detected using a luminescence assay. Less biofilm formation and decreased flagella motility halos were detected in the Cu-treated strain. Taken together, low-concentration of copper in the medium likely led to significant attenuation of multiple virulence factors of the SLTEC strain, providing new insights to the antimicrobial effect of copper.
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